BIOL202 Principles of Microbiology with Lab: What Fermentation is?

BIOL202 Principles of Microbiology with Lab: What Fermentation is?

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BIOL202 Principles of Microbiology with Lab

Questions:

Discussion:

Include a discussion of what fermentation is in addition to an explanation of your lab results & interpretations. How did we interpret our results? 

1. What is the purpose of adding a Durham tube to your culture? Does the Durham tube make this experiment selective or differential? 

2. Explain what occurs if your media turns pink in color. Is this a + or — result? 

3. Explain  what may have occurred if your media turns orange in color. Is this a + or — result? 

4. What are the possible end products of fermentation? Which ones are we detecting in this experiment? 

5. What is the purpose of the un-inoculated control in this test (we use a plain Lactose Broth)? 

7. List 2 indicators in this experiment? What are they detecting? 

8. Explain what fermentation is. What is the major benefit for an organism to be able to perform this metabolic process? 

9. Which monosaccharides are found in lactose? Which monosaccharides are found in sucrose? 

10. Can an organism grow in this broth and test negative for fermentation? 

11. What enzymes do the bacteria produce to hydrolyze sucrose and lactose into individual monosaccharides in the first step of fermentation? Why are they different? (•Hint: They are in the large flow chart for this experiment) 

BIOL202 Principles of Microbiology with Lab

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